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Abstract: Microinjection is a key method to animal gene transfer.
Ejection pressure, ejection duration, tip diameter of the pipette are
important factors that affect the dispensed volume of the
ultramicroinjector. The injection controllers currently used in this
field can’t accurately control the dispensed volume and thus lead to the
low injection efficiency and survival rate. A kind of electrical
controller microinjection controller with air as the pressure medium is
described. Based on the derivation of theoretical model the relationship
between dispensed volume and ejection pressure, ejection duration and
tip inner diameter is experimented and analyzed applying multiple factor
transformation regression design. The experiment results show that the
calibrated microinjection controller can satisfy the requirements of
gene transfer.
Key words: Gene transfer Ultramicroinjector controller Droplet
measurement Image processing system Multi-factor orthogonal
regression design
CLC No: H771
国家863计划(9914-10)及广东省重点学科(省970004)资助项目.
Received 20010416, received
in revised form 20010628
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